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Expression of microRNAs in plasma and in extracellular vesicles derived from plasma for dogs with glioma and dogs with other brain diseases.

脑胶质瘤犬和其他脑部疾病犬血浆和血浆来源的细胞外囊泡中 microrna 的表达。

  • 影响因子:1.20
  • DOI:10.2460/ajvr.81.4.355
  • 作者列表:"Narita M","Nishida H","Asahina R","Nakata K","Yano H","Dickinson PJ","Tanaka T","Akiyoshi H","Maeda S","Kamishina H
  • 发表时间:2020-04-01
Abstract

OBJECTIVE:To measure expression of microRNAs (miRNAs) in plasma and in extracellular vesicles (EVs) derived from plasma for dogs with glioma and dogs with other brain diseases. SAMPLE:Plasma samples from 11 dogs with glioma and 19 control dogs with various other brain diseases. PROCEDURES:EVs were isolated from plasma samples by means of ultracentrifugation. Expression of 4 candidate reference miRNAs (let-7a, miR-16, miR-26a, and miR-103) and 4 candidate target miRNAs (miR-15b, miR-21, miR-155, and miR-342-3p) was quantified with reverse transcription PCR assays. Three software programs were used to select the most suitable reference miRNAs from among the 4 candidate reference miRNAs. Expression of the 4 target miRNAs was then calculated relative to expression of the reference genes in plasma and EVs, and relative expression was compared between dogs with glioma and control dogs with other brain diseases. RESULTS:The most suitable reference miRNAs were miR-16 for plasma and let-7a for EVs. Relative expression of miR-15b in plasma and in EVs was significantly higher in dogs with glioma than in control dogs. Relative expression of miR-342-3p in EVs was significantly higher in dogs with glioma than in control dogs. CONCLUSIONS AND CLINICAL RELEVANCE:Results suggested that miR-15b and miR-342-3p have potential as noninvasive biomarkers for differentiating glioma from other intracranial diseases in dogs. However, more extensive analysis of expression in specific glioma subtypes and grades, compared with expression in more defined control populations, will be necessary to assess their clinical relevance.

摘要

目的: 检测脑胶质瘤犬和其他脑疾病犬血浆和血浆细胞外囊泡 (EVs) 中 microRNAs (miRNAs) 的表达。 样本: 11 只患有胶质瘤的犬和 19 只患有各种其他脑部疾病的对照犬的血浆样本。 程序: 通过超速离心法从血浆样品中分离 EVs。4 个候选参考 miRNAs (let-7a 、 miR-16 、 miR-26a 和 miR-103) 和 4 个候选靶 miRNAs (miR-15b 、 miR-21 、 miR-155 和 miR-342-3p) 的表达用逆转录 PCR 试验定量。使用 3 个软件程序从 4 个候选参考 miRNAs 中选择最合适的参考 miRNAs。然后计算 4 个靶 miRNAs 的表达相对于血浆和 EVs 中参考基因的表达,并比较胶质瘤犬和其他脑部疾病对照犬之间的相对表达。 结果: 血浆和 EVs miR-16 最合适的参考 miRNAs let-7a。脑胶质瘤犬血浆和 EVs 中 miR-15b 的相对表达量明显高于对照组犬。脑胶质瘤犬 EVs 中 miR-342-3p 的相对表达量明显高于对照组犬。 结论和临床相关性: 结果表明,miR-15b 和 miR-342-3p 有潜力作为非侵入性的生物标志物来鉴别胶质瘤和其他颅内疾病。然而,与在更明确的对照人群中表达相比,在特定胶质瘤亚型和分级中表达的更广泛分析将是必要的,以评估其临床相关性。

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影响因子:3.29
发表时间:2020-01-31
来源期刊:BMC cancer
DOI:10.1186/s12885-020-6536-x
作者列表:["Ang SYL","Lee L","See AAQ","Ang TY","Ang BT","King NKK"]

METHODS:BACKGROUND:Gliomas consist of a heterogeneous group of tumors. This study aimed to report the incidences of O6-methylguanine-DNA-methyltransferase (MGMT) promoter methylation, 1p19q co-deletion, isocitrate dehydrogenase (IDH) gene mutations, and inactivating mutations of alpha-thalassemia/mental retardation syndrome X-linked (ATRX) in high-grade gliomas in an ethnically diverse population. METHODS:Records of patients who underwent surgery for high-grade gliomas from January 2013 to March 2017 at our institution were obtained. The patients' age, gender, ethnicity, Karnofsky Performance Scale (KPS) score, ability to perform activities of daily living (ADLs), tumor location and biomarkers status were recorded. Data were analyzed using chi-square and Mann-Whitney U tests, Kaplan-Meier estimates and log-rank test. RESULTS:181 patients were selected (56 with grade III gliomas, 125 with grade IV gliomas). In the grade III group, 55% had MGMT promoter methylation, 41% had 1p19q co-deletion, 35% had IDH1 mutation and none had ATRX loss. In the grade IV group, 30% had MGMT promoter methylation, 2% had 1p19q co-deletion, 15% had IDH1 mutation and 8% had ATRX loss. After adjusting for effects of age, surgery and pre-operative ADL statuses, only MGMT promoter methylation was found to be significantly associated with longer overall survival time in grade III (p = 0.024) and IV patients (p = 0.006). CONCLUSIONS:The incidences of MGMT promoter methylation and IDH1 mutation were found to be comparable to globally reported rates, but those of 1p19q co-deletion and ATRX loss seemed to be lower in our cohort. MGMT promoter methylation was associated with increased overall survival in our cohort and might serve as favorable prognostic factor.

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影响因子:5.34
发表时间:2020-01-31
DOI:10.1186/s13046-020-1534-z
作者列表:["Abbruzzese C","Matteoni S","Persico M","Villani V","Paggi MG"]

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