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Vitamin D Suppresses Ovarian Cancer Growth and Invasion by Targeting Long Non-Coding RNA CCAT2.
维生素d 通过靶向长链非编码 RNA ccat2 抑制卵巢癌生长和侵袭。
- 影响因子:4.1830
- DOI:10.3390/ijms21072334
- 作者列表:"Wang L","Zhou S","Guo B
- 发表时间:2020-03-27
Abstract
:Ovarian cancer is the most deadly gynecologic cancer among women worldwide. Poor response to current treatment makes it necessary to discover new diagnostic biomarkers to detect the cancer early and develop new and effective prevention strategies. Calcitriol, the active metabolite of vitamin D, protects against multiple cancers through unelucidated mechanisms. The oncogenic long non-coding RNA (lncRNA) CCAT2 (colon cancer associated transcript 2) is overexpressed in ovarian cancer. Here, we foundd that calcitriol inhibited CCAT2 expression in ovarian cancer cell lines. Treatment with calcitriol inhibited ovarian cancer cell proliferation, migration, and invasion. As a result of CCAT2 inhibition, calcitriol decreased the binding of transcription factor TCF7L2 (TCF4) to the MYC promoter, resulting in the repression of c-Myc protein expression. Our results suggest a novel anti-cancer mechanism of vitamin D by targeting CCAT2 in ovarian cancer. The findings may help develop vitamin D as a practical and inexpensive nutraceutical for ovarian cancer prevention.
摘要
: 卵巢癌是全球女性中最致命的妇科癌症。对当前治疗反应不佳,有必要发现新的诊断生物标志物来早期发现癌症,并制定新的有效的预防策略。骨化三醇是维生素d 的活性代谢物,通过未阐明的机制预防多种癌症。致癌长链非编码 RNA (lncRNA) CCAT2 (结肠癌相关转录本 2) 在卵巢癌中过表达。在此,我们发现骨化三醇抑制卵巢癌细胞系 CCAT2 的表达。骨化三醇治疗可抑制卵巢癌细胞增殖、迁移和侵袭。由于 CCAT2 抑制,骨化三醇降低了转录因子 TCF7L2 (TCF4) 与 MYC 启动子的结合,导致 c-Myc 蛋白表达的抑制。我们的结果提示了维生素d 通过靶向 CCAT2 在卵巢癌中的抗癌机制。这些发现可能有助于开发维生素d 作为一种实用且廉价的预防卵巢癌的营养品。
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