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Long-lasting effects of antibiotics on bacterial communities of adult flies.
抗生素对成年果蝇细菌群落的长期影响。
- 影响因子:3.92
- DOI:10.1093/femsec/fiaa028
- 作者列表:"Ourry M","Lopez V","Hervé M","Lebreton L","Mougel C","Outreman Y","Poinsot D","Cortesero AM
- 发表时间:2020-04-01
Abstract
:Insect symbionts benefit their host and their study requires large spectrum antibiotic use like tetracycline to weaken or suppress symbiotic communities. While antibiotics have a negative impact on insect fitness, little is known about antibiotic effects on insect microbial communities and how long they last. We characterized the bacterial communities of adult cabbage root fly Delia radicum in a Wolbachia-free population and evaluated the effect of tetracycline treatment on these communities over several generations. Three D. radicum generations were used: the first- and second-generation flies either ingested tetracycline or not, while the third-generation flies were untreated but differed with their parents and/or grandparents that had or had not been treated. Fly bacterial communities were sequenced using a 16S rRNA gene. Tetracycline decreased fly bacterial diversity and induced modifications in both bacterial abundance and relative frequencies, still visible on untreated offspring whose parents and/or grandparents had been treated, therefore demonstrating long-lasting transgenerational effects on animal microbiomes after antibiotic treatment. Flies with an antibiotic history shared bacterial genera, potentially tetracycline resistant and heritable. Next, the transmission should be investigated by comparing several insect development stages and plant compartments to assess vertical and horizontal transmissions of D. radicum bacterial communities.
摘要
: 昆虫共生体对宿主有益,他们的研究需要像四环素一样使用广谱抗生素来削弱或抑制共生群落。虽然抗生素对昆虫的适应性有负面影响,但人们对抗生素对昆虫微生物群落的影响及其持续时间知之甚少。我们在无沃尔巴克氏体种群中表征了成年甘蓝根蝇的细菌群落,并评价了四环素处理对这些群落几代的影响。三个 D.使用 radicum 世代: 第一代和第二代果蝇摄入或不摄入四环素,而第三代果蝇未经治疗,但与他们的父母和/或祖父母不同,他们已经或没有接受治疗。使用 16S rRNA 基因对蝇类细菌群落进行测序。四环素降低了蝇类细菌多样性,并诱导了细菌丰度和相对频率的改变,在父母和/或祖父母接受治疗的未经治疗的后代上仍然可见,因此证明了抗生素治疗后对动物微生物组的持久跨代效应。具有抗生素历史的苍蝇共有细菌属,可能对四环素耐药且可遗传。接下来,应通过比较几个昆虫发育阶段和植物区室来研究传播,以评估 D. radicum 细菌群落的垂直和水平传播。
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