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Prevention of excessive exercise-induced adverse effects in rats with Bacillus subtilis BSB3.

枯草芽孢杆菌BSB3对大鼠过度运动所致不良反应的预防作用。

  • 影响因子:2.72
  • DOI:10.1111/jam.14544
  • 作者列表:"Ducray HAG","Globa L","Pustovyy O","Roberts MD","Rudisill M","Vodyanoy V","Sorokulova I
  • 发表时间:2020-04-01
Abstract

AIMS:To characterize efficacy of the Bacillus subtilis BSB3 (BSB3) strain in the prevention of excessive exercise-induced side effects and in maintaining stability of the gut microbiota. METHODS AND RESULTS:Rats were pretreated by oral gavage with B. subtilis BSB3 (BSB3) or with phosphate-buffered saline (PBS) twice a day for 2 days, and were either exposed forced treadmill running or remained sedentary. Histological analysis of intestine, immunofluorescence staining of tight junction (TJ) proteins, serum lipopolysaccharide and intestinal fatty acid-binding protein assay, culture-based analysis and pyrosequencing for the gut microbiota were performed for each rat. Forced running resulted in a substantial decrease in intestinal villi height and total mucosa thickness, the depletion of Paneth cells, an inhibition of TJ proteins expression. Short-term treatment of rats with BSB3 before running prevented these adverse effects. Culture-based analysis of the gut microbiota revealed significant elevation of pathogenic microorganisms only in treadmill-exercised rats pretreated with PBS. High-throughput 16S rRNA gene sequencing also revealed an increase in pathobionts in this group. Preventive treatment of animals with BSB3 resulted in predominance of beneficial bacteria. CONCLUSIONS:BSB3 prevents excessive exercise-associated complications by beneficial modulation of the gut microbiota. SIGNIFICANCE AND IMPACT OF THE STUDY:Our study shows a new application of beneficial bacteria for prevention the adverse effects of excessive exercise.

摘要

目的: 表征枯草芽孢杆菌 BSB3 (BSB3) 菌株在预防过度运动引起的副作用和维持肠道菌群稳定方面的功效。 方法和结果: 大鼠经口灌胃 B.枯草杆菌 BSB3 (BSB3) 或用磷酸盐缓冲盐水 (PBS),每天两次,持续 2 天,要么暴露于强制跑台,要么保持久坐不动。肠组织学分析,紧密连接 (TJ) 蛋白免疫荧光染色,血清脂多糖和肠脂肪酸结合蛋白测定,对每只大鼠进行基于培养的分析和肠道菌群的焦磷酸测序。强迫跑步导致肠绒毛高度和总粘膜厚度大幅下降,潘氏细胞耗竭,抑制 TJ 蛋白表达。跑步前用 BSB3 短期治疗大鼠可预防这些不良反应。基于培养的肠道菌群分析显示,仅在用 PBS 预处理的跑台运动大鼠中,病原微生物显著升高。高通量 16S rRNA 基因测序也揭示了该组中致病基因的增加。用 BSB3 对动物进行预防性治疗,导致有益菌占优势。 结论: BSB3 通过有益的肠道菌群调节防止过度运动相关的并发症。 研究的意义和影响: 我们的研究显示了有益细菌的新应用,用于预防过度运动的不良影响。

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发表时间:2020-01-21
DOI:10.1093/ibd/izz331
作者列表:["Ronchetti S","Gentili M","Ricci E","Migliorati G","Riccardi C"]

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关键词: GILZ IBD 自身免疫 炎症
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