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Long Noncoding RNA FENDRR Exhibits Antifibrotic Activity in Pulmonary Fibrosis.

长链非编码 RNA FENDRR 在肺纤维化中表现出抗纤维化活性。

  • 影响因子:3.37
  • DOI:10.1165/rcmb.2018-0293OC
  • 作者列表:"Huang C","Liang Y","Zeng X","Yang X","Xu D","Gou X","Sathiaseelan R","Senavirathna LK","Wang P","Liu L
  • 发表时间:2020-04-01
Abstract

:Abnormal activation of lung fibroblasts contributes to the initiation and progression of idiopathic pulmonary fibrosis (IPF). The objective of the present study was to investigate the role of fetal-lethal noncoding developmental regulatory RNA (FENDRR) in the activation of lung fibroblasts. Dysregulated long noncoding RNAs in IPF lungs were identified by next-generation sequencing analysis from the two online datasets. FENDRR expression in lung tissues from patients with IPF and mice with bleomycin-induced pulmonary fibrosis was determined by quantitative real-time PCR. IRP1 (iron-responsive element-binding protein 1), a protein partner of FENDRR, was identified by RNA pulldown-coupled mass spectrometric analysis and confirmed by RNA immunoprecipitation. The interaction region between FENDRR and IRP1 was determined by cross-linking immunoprecipitation. The in vivo role of FENDRR in pulmonary fibrosis was studied using adenovirus-mediated gene transfer in mice. The expression of FENDRR was downregulated in fibrotic human and mouse lungs as well as in primary lung fibroblasts isolated from bleomycin-treated mice. TGF-β1 (transforming growth factor-β1)-SMAD3 signaling inhibited FENDRR expression in lung fibroblasts. FENDRR was preferentially localized in the cytoplasm of adult lung fibroblasts and bound IRP1, suggesting its role in iron metabolism. FENDRR reduced pulmonary fibrosis by inhibiting fibroblast activation by reducing iron concentration and acting as a competing endogenous RNA of the profibrotic microRNA-214. Adenovirus-mediated FENDRR gene transfer in the mouse lung attenuated bleomycin-induced lung fibrosis and improved lung function. Our data suggest that FENDRR is an antifibrotic long noncoding RNA and a potential therapeutic target for pulmonary fibrosis.

摘要

: 肺成纤维细胞的异常活化有助于特发性肺纤维化 (IPF) 的启动和进展。本研究的目的是探讨胎儿致死性非编码发育调节 RNA (FENDRR) 在肺成纤维细胞活化中的作用。通过来自两个在线数据集的新一代测序分析鉴定了 IPF 肺中失调的长链非编码 rna。通过实时定量 PCR 测定 IPF 患者和博莱霉素诱导的肺纤维化小鼠肺组织中 FENDRR 的表达。通过 RNA 下拉偶联质谱分析鉴定了 FENDRR 的蛋白伴侣 IRP1 (铁反应元件结合蛋白 1),并通过 RNA 免疫沉淀进行了确认。通过交联免疫沉淀确定 FENDRR 与 IRP1 的相互作用区域。用腺病毒介导的小鼠基因转移研究了 FENDRR 在肺纤维化中的体内作用。FENDRR 的表达在纤维化的人和小鼠肺以及从博莱霉素处理的小鼠中分离的原代肺成纤维细胞中下调。Tgf-β 1 (转化生长因子-β 1)-SMAD3 信号抑制肺成纤维细胞 FENDRR 表达。FENDRR 优先定位于成人肺成纤维细胞的细胞质中,并结合 IRP1,提示其在铁代谢中的作用。FENDRR 通过降低铁浓度抑制成纤维细胞活化,并作为促纤维化 microRNA-214 的竞争性内源性 RNA,从而减轻肺纤维化。腺病毒介导的小鼠肺 FENDRR 基因转移可减轻博莱霉素诱导的肺纤维化,改善肺功能。我们的数据表明 FENDRR 是一种抗纤维化长链非编码 RNA,是肺纤维化的潜在治疗靶点。

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翻译标题与摘要 下载文献
影响因子:4.40
发表时间:2020-01-01
DOI:10.1007/s00262-019-02431-8
作者列表:["Shibaki, Ryota","Murakami, Shuji","Matsumoto, Yuji","Yoshida, Tatsuya","Goto, Yasushi","Kanda, Shintaro","Horinouchi, Hidehito","Fujiwara, Yutaka","Yamamoto, Nobuyuki","Kusumoto, Masahiko","Yamamoto, Noboru","Ohe, Yuichiro"]

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翻译标题与摘要 下载文献
影响因子:4.04
发表时间:2020-01-25
来源期刊:New biotechnology
DOI:10.1016/j.nbt.2019.08.006
作者列表:["Sousa SA","Soares-Castro P","Seixas AMM","Feliciano JR","Balugas B","Barreto C","Pereira L","Santos PM","Leitão JH"]

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