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Acute effects of e-cigarette vaping on pulmonary function and airway inflammation in healthy individuals and in patients with asthma.

电子烟汽化对健康人和哮喘患者肺功能和气道炎症的急性影响。

  • 影响因子:3.15
  • DOI:10.1111/resp.13806
  • 作者列表:"Kotoulas SC","Pataka A","Domvri K","Spyratos D","Katsaounou P","Porpodis K","Fouka E","Markopoulou A","Passa-Fekete K","Grigoriou I","Kontakiotis T","Argyropoulou P","Papakosta D
  • 发表时间:2020-04-02
Abstract

BACKGROUND AND OBJECTIVE:The acute effects of e-cigarettes have not been scientifically demonstrated yet. The aim of this study was to assess the acute changes in pulmonary function and airway inflammation in patients with asthma after vaping one e-cigarette. METHODS:Twenty-five smokers suffering from stable moderate asthma according to GINA guidelines with no other comorbidities and 25 healthy smokers matched with the baseline characteristics of the asthmatic patients were recruited. PFT, IOS, FeNO and EBC were performed before and after vaping one e-cigarette with nicotine. pH and concentrations of IL-1β, IL-4, IL-5, IL-6, IL-8, IL-10, IL-13, IL-17A, TNF-α, ISO8 and LTB4 were measured in EBC. RESULTS:FFEV1/FVC ratio and PEF were reduced in asthmatic patients after e-cigarette. Z5Hz and R5Hz, R10Hz and R20Hz increased in both groups. FeNO and EBC pH increased by 3.60 ppb (P = 0.001) and 0.15 (P = 0.014) in asthmatic patients after e-cigarette, whereas they decreased in control group by 3.28 ppb (P < 0.001) and 0.12 (P = 0.064), respectively. The concentrations of IL-10, TNF-α and ISO8 in EBC increased in asthmatic patients after e-cigarette and the changes in concentrations of IL-1β and IL-4 differed significantly between the two groups. CONCLUSION:E-cigarette vaping resulted in acute alteration of both pulmonary function and airway inflammation in stable moderate asthmatic patients.

摘要

背景和目的: 电子烟的急性效应尚未得到科学证明。本研究的目的是评估哮喘患者在吸一根电子烟后肺功能和气道炎症的急性变化。 方法: 根据 GINA 指南,25 例患有稳定中度哮喘且无其他合并症的吸烟者和 25 例符合哮喘患者基线特征的健康吸烟者。PFT 、 IOS 、 FeNO 和 EBC 在用尼古丁汽化一根电子烟之前和之后进行。测定 EBC 中 il-1 β 、 IL-4 、 IL-5 、 IL-6 、 IL-8 、 IL-10 、 IL-13 、 IL-17A 、、 TNF-α 、 ISO8 和 LTB4 的浓度。 结果: 电子烟后哮喘患者 FFEV1/FVC 比值和 PEF 降低。Z5Hz 和 R5Hz,R10Hz 和 R20Hz 两组均有所增加。电子烟后哮喘患者 FeNO 和 EBC pH 分别增加 3.60 ppppb (P = 0.001) 和 0.15 (P = 0.014),而对照组分别降低 3.28 ppb (P <0.001) 和 0.12 (P = 0.064)。电子烟后哮喘患者 EBC 中 IL-10 、 TNF-α 和 ISO8 浓度升高,两组间 il-1 β 和 IL-4 浓度变化差异有统计学意义。 结论: 电子烟雾化吸入可导致稳定中度哮喘患者肺功能和气道炎症的急性改变。

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影响因子:3.94
发表时间:2020-01-15
DOI:10.1016/j.taap.2019.114847
作者列表:["Bernstein DM","Toth B","Rogers RA","Kling DE","Kunzendorf P","Phillips JI","Ernst H"]

METHODS::The interim results from this 90-day multi-dose, inhalation toxicology study with life-time post-exposure observation has shown an important fundamental difference in persistence and pathological response in the lung between brake dust derived from brake-pads manufactured with chrysotile, TiO2 or chrysotile alone in comparison to the amphiboles, crocidolite and amosite asbestos. In the brake dust exposure groups no significant pathological response was observed at any time. Slight macrophage accumulation of particles was noted. Wagner-scores, were from 1 to 2 (1 = air-control group) and were similar to the TiO2 group. Chrysotile being biodegradable, shows a weakening of its matrix and breaking into short fibers & particles that can be cleared by alveolar macrophages and continued dissolution. In the chrysotile exposure groups, particle laden macrophage accumulation was noted leading to a slight interstitial inflammatory response (Wagner-score 1-3). There was no peribronchiolar inflammation and occasional very slight interstitial fibrosis. The histopathology and the confocal analyses clearly differentiate the pathological response from amphibole asbestos, crocidolite and amosite, compared to that from the brake dust and chrysotile. Both crocidolite and amosite induced persistent inflammation, microgranulomas, and fibrosis (Wagner-scores 4), which persisted through the post exposure period. The confocal microscopy of the lung and snap-frozen chestwalls quantified the extensive inflammatory response and collagen development in the lung and on the visceral and parietal surfaces. The interim results reported here, provide a clear basis for differentiating the effects from brake dust exposure from those following amphibole asbestos exposure. The subsequent results through life-time post-exposure will follow.

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影响因子:4.04
发表时间:2020-01-10
DOI:10.1042/BST20191010
作者列表:["Zaragosi LE","Deprez M","Barbry P"]

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