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TET2 promotor methylation and TET2 protein expression in pediatric posterior fossa ependymoma.

TET2 启动子甲基化和 TET2 蛋白在儿童后颅窝室管膜瘤中的表达。

  • 影响因子:2.09
  • DOI:10.1111/neup.12615
  • 作者列表:"Pierscianek D","Teuber-Hanselmann S","Ahmadipour Y","Darkwah Oppong M","Unteroberdörster M","Müller O","Jabbarli R","Sure U","Zhu Y","El Hindy N
  • 发表时间:2020-04-01
Abstract

Pediatric posterior fossa ependymoma (PF) is one of the most common brain tumors in children. Recently, two subtypes of PF were identified. PF-A has a dismal prognosis and shows a hypermethylation phenotype, whereas PF-B shows a great genomic instability. The ten-eleven translocation methylcytosine dioxygenase 2 (TET2) gene (TET2) has been linked to the regulation of DNA methylation. We analyzed TET2 promotor methylation and protein expression to assess the role of TET2 in PF. Medical records of all PF cases treated in our institution between 1993 and 2015 were evaluated regarding tumor histology, grade, tumor location, gender, age, tumor recurrence, distant metastasis, survival and time to progression. Subsequently, we analyzed TET2 promotor methylation using methylation-specific polymerase chain reaction. TET2 protein expression was assessed using immunohistochemistry. Low TET2 expression was detected in seven of 17 cases. There was an association between low TET2 expression and tumor recurrence (P = 0.049). A TET2 promotor methylation was detected in five of 10 cases. There was no association between the TET2 promotor methylation with recurrence, tumor grade or gender. TET2 promotor methylation and low TET2 expression was detected in a subgroup of PF. Our data show an association between low TET2 expression and tumor recurrence in PF.

摘要

小儿后颅窝室管膜瘤 (PF) 是儿童最常见的脑肿瘤之一。最近发现了 PF 的两种亚型。PF-A 预后不佳,表现出高甲基化表型,而 PF-B 表现出很大的基因组不稳定性。十一易位甲基胞嘧啶双加氧酶 2 (TET2) 基因 (TET2) 与 DNA 甲基化的调控有关。我们分析了 TET2 启动子甲基化和蛋白表达,以评估 TET2 在 PF 中的作用。对 2015 和 1993年在本机构治疗的所有 PF 病例的病历进行了肿瘤组织学、分级、肿瘤部位、性别、年龄、肿瘤复发、远处转移、生存期和进展时间的评价。随后,我们使用甲基化特异性聚合酶链反应分析 TET2 启动子甲基化。使用免疫组织化学评估 TET2 蛋白表达。17 例中 7 例检测到 TET2 低表达。TET2 低表达与肿瘤复发有相关性 (P = 0.049)。10 例中有 5 例检测到 TET2 启动子甲基化。TET2 启动子甲基化与复发、肿瘤分级或性别之间无相关性。在 PF 亚组中检测到 TET2 启动子甲基化和 TET2 低表达。我们的数据显示了 PF 中 TET2 低表达与肿瘤复发之间的相关性。

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