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Molecular and epidemiological trends of human bocavirus and adenovirus in children with acute gastroenteritis in Bangladesh during 2015-2019.

2015-2019 年孟加拉国急性胃肠炎儿童中人博卡病毒和腺病毒的分子和流行病学趋势。

  • 影响因子:1.94
  • DOI:10.1002/jmv.25812
  • 作者列表:"Sharif N","Parvez AK","Haque A","Talukder AA","Ushijima H","Dey SK
  • 发表时间:2020-04-01
Abstract

Virus associated diarrhea remains one of the leading causes of children morbidity and mortality in Bangladesh. Human bocavirus (HBoV) has been reported as a potential pathogen of children diarrhea worldwide. However, due to its frequent association with other gastroenteric pathogens its role as diarrhea causative agent remains to be defined. This study focuses to detect the incidence of HBoV and adenovirus (AdV) and to determine molecular and epidemiological characteristics of HBoV and AdV. Between January 2015 to January 2019, 290 fecal specimens were collected from diarrheal children in Bangladesh. All fecal specimens were tested for HBoV and AdV by conventional polymerase chain reaction and sequencing methods. HBoV was detected in 7.24% (21 of 290) of the stool samples, as a sole virus in 71.42% (15 of 21) of the positive samples. Adenovirus was detected in 4.82% (14 of 290) of the samples. The most common clinical symptoms of HBoV infected patients were diarrhea (100%) and vomiting (57%). All of the isolates of HBoV were from HBoV1 and AdV were from AdV41, AdV5, AdV7 & AdV8. To the best of our knowledge this is the first epidemiological and molecular analysis report of HBoV from clinical specimens in Bangladesh. In future more studies are needed to clarify the role of HBoV as diarrheal pathogens. This article is protected by copyright. All rights reserved.

摘要

病毒相关性腹泻仍然是孟加拉国儿童发病和死亡的主要原因之一。人类博卡病毒 (HBoV) 已被报道为全球儿童腹泻的潜在病原体。然而,由于其经常与其他胃肠道病原体相关,其作为腹泻病原体的作用仍有待确定。本研究旨在检测 HBoV 和腺病毒 (AdV) 的发病率,并确定 HBoV 和 AdV 的分子和流行病学特征。在 2015年1月至 2019年1月期间,收集了 290 份来自孟加拉国腹泻儿童的粪便标本。所有粪便标本均采用常规聚合酶链反应和测序方法检测 HBoV 和 AdV。7.24% (290) 的粪便样本检出 HBoV,71.42% (21) 的阳性样本检出 HBoV 为单一病毒。4.82% (290 份中的 14 份) 的样本检测到腺病毒。HBoV 感染患者最常见的临床症状为腹泻 (100%) 和呕吐 (57%)。HBoV 的分离株均来自 HBoV1,AdV 分别来自 AdV41 、 AdV5 、 AdV7 和 adv8。据我们所知,这是孟加拉国第一份来自临床标本的 HBoV 流行病学和分子分析报告。未来还需要更多的研究来阐明 HBoV 作为腹泻病原体的作用。本文受版权保护。保留所有权利。

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来源期刊:mBio
DOI:10.1128/mBio.03105-19
作者列表:["Kuehl CJ","D'Gama JD","Warr AR","Waldor MK"]

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影响因子:5.36
发表时间:2020-01-20
DOI:10.1007/s00259-020-04686-1
作者列表:["Willowson KP","Schembri GP","Bernard EJ","Chan DL","Bailey DL"]

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影响因子:3.32
发表时间:2020-01-09
DOI:10.1016/j.intimp.2019.106144
作者列表:["Fan M","Xiang G","Chen J","Gao J","Xue W","Wang Y","Li W","Zhou L","Jiao R","Shen Y","Xu Q"]

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