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microRNA-217 suppressed epithelial-to-mesenchymal transition through targeting PTPN14 in gastric cancer.

MicroRNA-217 通过靶向 PTPN14 抑制胃癌上皮间质转化。

  • 影响因子:2.87
  • DOI:10.1042/BSR20193176
  • 作者列表:"Chen G","Yang Z","Feng M","Wang Z
  • 发表时间:2020-01-31
Abstract

BACKGROUND:Gastric cancer (GC) is the one of most common malignancies and its mechanism of metastasis remains unclear. The study was designed to investigate the effects of microRNA-217 on epithelial-to-mesenchymal transition. METHODS:The expression levels of miR-217 in GC were assayed by real-time qPCR. Metastasis and invasion of cancer cell were assayed by transwell chamber. Double luciferase reporter gene was used to verify the target regulatory relationship between microRNA-217 and tyrosine-protein phosphatase non-receptor type 14 (PTPN14) on gastric cell lines. Epithelial-to-mesenchymal transition (EMT) markers were assayed by Western blot. RESULTS:We found that miR-217 had a low level expression in gastric tumor tissues of 40 patients with GC, and a lower expression in the gastric tumor tissues of the patients with GC metastasis. Moreover, miR-217 markedly suppressed the metastasis and invasion of gastric cancer cell line in vitro. Furthermore, miR-217 inhibited the expression of PTPN14 by directly targeting its 3'UTR. Moreover, the down-regulation of PTPN14 reduced the metastasis and invasion, whereas up-regulation of PTPN14 led to the enhanced metastases and invasion of gastric cells. miR-217 induced the down-regulation of PTPN14 and inhibited the EMT in gastric cancer cells. CONCLUSION:miR-217 inhibited the EMT through directly targeting to the 3'UTR of PTPN14.

摘要

背景: 胃癌是最常见的恶性肿瘤之一,其转移机制尚不清楚。本研究旨在探讨 microRNA-217 对上皮间质转化的影响。 方法: 采用实时荧光定量 pcr 法检测 GC 中 miR-217 的表达水平。Transwell 小室检测癌细胞的转移和侵袭。利用双荧光素酶报告基因验证 microRNA-217 与胃细胞系上酪氨酸-蛋白磷酸酶非受体 14 型 (PTPN14) 的靶调控关系。Western blot 检测上皮间质转化 (EMT) 标志物。 结果: 我们发现 miR-217 在 40 例胃癌患者的胃肿瘤组织中呈低水平表达,在胃癌转移患者的胃肿瘤组织中呈低水平表达。MiR-217 能明显抑制胃癌细胞的体外转移和侵袭。此外,miR-217 通过直接靶向其 3 'utr 抑制 PTPN14 的表达。此外,PTPN14 的下调减少了胃细胞的转移和侵袭,而 PTPN14 的上调导致了胃细胞转移和侵袭的增强。 miR-217 诱导 PTPN14 表达下调,抑制胃癌细胞 EMT。 结论: miR-217 通过直接靶向 ptpn14 的 3 'utr 抑制 EMT。

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影响因子:1.71
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作者列表:["Jing JJ","Li H","Wang ZY","Zhou H","Sun LP","Yuan Y"]

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发表时间:2020-01-28
DOI:10.1002/jcp.29562
作者列表:["Daryabari SS","Fathi M","Mahdavi M","Moaddab Y","Hosseinpour Feizi MA","Shokoohi B","Safaralizadeh R"]

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