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Overexpression of CFL1 in gastric cancer and the effects of its silencing by siRNA with a nanoparticle delivery system in the gastric cancer cell line.

CFL1 在胃癌中的过表达及其在胃癌细胞系中通过 siRNA 与纳米颗粒递送系统沉默的影响。

  • 影响因子:3.89
  • DOI:10.1002/jcp.29562
  • 作者列表:"Daryabari SS","Fathi M","Mahdavi M","Moaddab Y","Hosseinpour Feizi MA","Shokoohi B","Safaralizadeh R
  • 发表时间:2020-01-28
Abstract

Gastric adenocarcinoma, like other cancers, is a multifactorial genetic disease, andmetastasis of cancer cells is one of the main features of this illness. The expressionlevels of the CFL1 gene have been modulated in this pathway. Using small interferingRNA (siRNA) in the treatment of gastric cancer is considered a hopeful genetherapeutic approach. The present study reported the level of CFL1 genes betweentumor and margin and healthy tissue of gastric cancer. Also, the features of a cationicnanoparticle with a polymer coating containing polyacrylic acid and polyethylenei-mine that were used in the delivery of CFL1 siRNA, were shown. Then thecytotoxicity, cellular uptake, and gene silencing efficiency of this nanoparticle wereevaluated with CFL1siRNA. Method:In this study, the CFL1 gene expression was measured in 40 gastricadenocarcinoma, marginal and 15 healthy biopsy samples by a real‐time polymerasechain reaction. Physicochemical characteristics, apoptosis, and inhibition of migrationof the delivery of CFL1 siRNA by nanoparticle and lipofectamine were investigated ingastric cancer cells. Result:The CFL1 expression was remarkably increased in gastric cancer tissues incomparison with the marginal samples and normal tissues (p< .05) and the biomarkerindex for CFL1 was obtained as 0.94, then this gene can be probably used as abiomarker for gastric cancer. After treatment of the AGS cell line by CFL1 siRNA, theCFL1 expression level of mRNA and migration in AGS cells were remarkablysuppressed after transfection. Furthermore, the amount of apoptosis increased(p< .05). Conclusion:Our results demonstrated that CFL1 downregulation in AGS cells caninterdict cell migration. Finally, our outcomes propose that CFL1 can function as anoncogenic gene in gastric cancer and would be considered as a potential purpose ofgene therapy for gastric cancer treatment

摘要

胃腺癌与其他癌症一样,是一种多因素遗传病,癌细胞转移是其主要特征之一。胃腺癌的表达水平与其他癌症一样,是一种多因素的遗传病,癌细胞转移是其主要特征之一。CFL1基因的表达水平在这一途径中被调控。用小干扰rna(siRNA)治疗胃癌被认为是一种有希望的基因治疗方法。本文报道了胃癌癌旁组织和健康组织中CFL1基因的表达水平。此外,还展示了用于递送CFL1 siRNA的含有聚丙烯酸和聚乙烯亚胺的聚合物涂层的阳离子纳米粒子的特征。 方法:采用实时聚合酶链反应检测40例胃粘膜癌、15例健康活检组织和边缘组织中CFL1基因的表达。理化特性,细胞凋亡,结果:与癌旁组织和正常组织相比,胃癌组织中CFL1的表达显著增加(p<0.05),CFL1的生物标记指数为0.94,表明该基因在胃癌细胞中的表达明显高于癌旁组织和正常组织(p<0.05)可能是胃癌的标志物。CFL1 siRNA处理AGS细胞后,转染AGS细胞后,其mRNA的表达水平和迁移率显著降低。 结论:CFL1在AGS细胞中的表达下调,可导致细胞迁移。最后,我们的研究结果提示CFL1在胃癌中可以作为一个非编码基因发挥作用,并被认为是胃癌基因治疗的一个潜在目的CFL1基因在这一途径中被调控。用小干扰rna(siRNA)治疗胃癌被认为是一种有希望的基因治疗方法。本文报道了胃癌癌旁组织和健康组织中CFL1基因的表达水平。此外,还展示了用于递送CFL1 siRNA的含有聚丙烯酸和聚乙烯亚胺的聚合物涂层的阳离子纳米粒子的特征。然后用CFL1siRNA对该纳米颗粒的细胞毒性、细胞摄取和基因沉默效率进行评价。方法:本研究通过实时聚合酶链反应,测定了40例胃粘膜癌、边缘癌和15例健康活检组织中CFL1基因的表达。理化特性,细胞凋亡,结果:与癌旁组织和正常组织相比,胃癌组织中CFL1的表达显著增加(p<0.05),CFL1的生物标记指数为0.94,表明该基因在胃癌细胞中的表达明显高于癌旁组织和正常组织(p<0.05)可能是胃癌的标志物。CFL1 siRNA处理AGS细胞后,转染AGS细胞后,其mRNA的表达水平和迁移率显著降低。 结论:CFL1在AGS细胞中的表达下调,可导致细胞迁移。最后,我们的研究结果提示CFL1在胃癌中可以作为一个非致癌基因发挥作用,并被认为是胃癌基因治疗的潜在目的。

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影响因子:3.43
发表时间:2020-01-28
DOI:10.1002/mc.23158
作者列表:["Xie W","Chen C","Han Z","Huang J","Liu X","Chen H","Zhang T","Chen S","Chen C","Lu M","Shen X","Xue X"]

METHODS::Diffuse gastric cancer (DGC) is a lethal malignancy lacking effective systemic therapy. Among the most provocative recent results in DGC has been that the alter of the cellular cytoskeleton and intercellular adhesion. CD2-associated protein (CD2AP) is one of the critical proteins regulating cytoskeleton assembly and intercellular adhesion. However, no study has investigated the expression and biological significance of CD2AP in gastric cancer (GC) to date. Therefore, the aim of our study was to explore if the expression of CD2AP is associated with any clinical features of GC and to elucidate the underlying mechanism. Immunohistochemistry of 620 patient tissue samples indicated that the expression of CD2AP is downregulated in DGC. Moreover, a low CD2AP level was indicative of poor patient prognosis. In vitro, forced expression of CD2AP caused a significant decrease in the migration and invasion of GC cells, whereas depletion of CD2AP had the opposite effect. Immunofluorescence analysis indicated that CD2AP promoted cellular adhesion and influenced cell cytoskeleton assembly via interaction with the F-actin capping protein CAPZA1. Overall, the upregulation of CD2AP could attenuate GC metastasis, suggesting CD2AP as a novel biomarker for the prognosis and treatment of patients with GC.

翻译标题与摘要 下载文献
影响因子:1.71
发表时间:2020-01-28
DOI:10.2217/fon-2019-0649
作者列表:["Jing JJ","Li H","Wang ZY","Zhou H","Sun LP","Yuan Y"]

METHODS::Aim: To identify the methylated-differentially expressed genes (MDEGs) that may serve as diagnostic markers and therapeutic targets in Epstein-Barr virus-associated gastric cancer (EBVaGC) and to explore the methylation-based pathways for elucidating biological mechanisms of EBVaGC. Materials & methods: Gene expression and methylation profiles were downloaded from GEO database. MDEGs were identified by GEO2R. Pathway enrichment analyses were conducted based on DAVID database. Hub genes were identified by Cytoscape, which were further verified by The Cancer Genome Atlas database. Results: A total of 367 hypermethylated, lowly expressed genes were enriched in specific patterns of cell differentiation. 31 hypomethylated, highly expressed genes demonstrated enrichment in regulation of immune system process. After validation using The Cancer Genome Atlas database, seven genes were confirmed to be significantly different hub genes in EBVaGC. Conclusion: EBVaGC-specific MDEGs and pathways can be served as potential biomarkers for precise diagnosis and treatment of EBVaGC and provide novel insights into the mechanisms involved.

翻译标题与摘要 下载文献
影响因子:3.89
发表时间:2020-01-28
DOI:10.1002/jcp.29562
作者列表:["Daryabari SS","Fathi M","Mahdavi M","Moaddab Y","Hosseinpour Feizi MA","Shokoohi B","Safaralizadeh R"]

METHODS:Gastric adenocarcinoma, like other cancers, is a multifactorial genetic disease, andmetastasis of cancer cells is one of the main features of this illness. The expressionlevels of the CFL1 gene have been modulated in this pathway. Using small interferingRNA (siRNA) in the treatment of gastric cancer is considered a hopeful genetherapeutic approach. The present study reported the level of CFL1 genes betweentumor and margin and healthy tissue of gastric cancer. Also, the features of a cationicnanoparticle with a polymer coating containing polyacrylic acid and polyethylenei-mine that were used in the delivery of CFL1 siRNA, were shown. Then thecytotoxicity, cellular uptake, and gene silencing efficiency of this nanoparticle wereevaluated with CFL1siRNA. Method:In this study, the CFL1 gene expression was measured in 40 gastricadenocarcinoma, marginal and 15 healthy biopsy samples by a real‐time polymerasechain reaction. Physicochemical characteristics, apoptosis, and inhibition of migrationof the delivery of CFL1 siRNA by nanoparticle and lipofectamine were investigated ingastric cancer cells. Result:The CFL1 expression was remarkably increased in gastric cancer tissues incomparison with the marginal samples and normal tissues (p< .05) and the biomarkerindex for CFL1 was obtained as 0.94, then this gene can be probably used as abiomarker for gastric cancer. After treatment of the AGS cell line by CFL1 siRNA, theCFL1 expression level of mRNA and migration in AGS cells were remarkablysuppressed after transfection. Furthermore, the amount of apoptosis increased(p< .05). Conclusion:Our results demonstrated that CFL1 downregulation in AGS cells caninterdict cell migration. Finally, our outcomes propose that CFL1 can function as anoncogenic gene in gastric cancer and would be considered as a potential purpose ofgene therapy for gastric cancer treatment

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