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Expression assessment of a panel of long non-coding RNAs in gastric malignancy.
一组长链非编码 rna 在胃恶性肿瘤中的表达评估。
- 影响因子：2.39
- DOI：10.1016/j.yexmp.2020.104383
- 作者列表："Esfandi F","Salehnezhad T","Taheri M","Afsharpad M","Hafez AA","Oskooei VK","Ghafouri-Fard S
- 发表时间：2020-01-23
Abstract
BACKGROUND:Long non-coding RNAs (lncRNAs) have several important functions in the regulation of cell homeostasis and cell fate. Consequently, abnormal transcription of lncRNAs has been correlated with malignant transformation of cells. These human transcripts have been shown to participate in the progression of gastric cancer. METHODS:In the current project, we evaluated expression of a panel of lncRNAs including HULC, MALAT1, FAS-AS1, GAS5, PVT1, OIP5-AS1 and THRIL in 30 gastric cancer tissues and paired adjacent non-cancerous tissues (ANCTs) using quantitative real-time PCR. RESULTS:HULC, OIP5-AS1 and THRIL transcription quantities were significantly lower in gastric tumors compared to ANCTs (P values = .02, 0.02 and 0.007, respectively). Relative transcription quantities of HULC, MALAT1, OIP5-AS1, PVT1, FAS-AS1 and THRIL were associated with the site of the primary tumor (P values = .002, 0.003, 0.002, 0.002, 0.002, and 0.001, respectively). Moreover, relative expression levels of PVT1 were associated with history of smoking (P value = .04). Correlations were identified between transcript quantities of these lncRNAs in both tumor samples and ANCTs. Receiver operating characteristic curve assessment demonstrated that THRIL had the highest diagnostic power among the mentioned lncRNAs (area under curve (AUC) = 0.72, P value = .001). HULC and OIP5-AS1 ranked afterwards (AUC values of 0.69 and 0.68; P values = .005 and 0.007, respectively). CONCLUSION:The current investigation underscores the dysregulation of these transcripts in gastric cancer specimens and suggests a number of these transcripts for further assessments of their suitability as cancer biomarkers.
摘要
背景: 长链非编码 rna (Long non-coding RNAs，lncRNAs) 在调节细胞稳态和细胞命运方面有几个重要功能。因此，lncRNAs 的异常转录与细胞的恶性转化相关。这些人类转录本已被证明参与胃癌的进展。 方法: 在当前项目中，我们评估了一组 lncRNAs 的表达，包括 HULC 、 MALAT1 、 FAS-AS1 、 GAS5 、 PVT1 、采用实时定量 PCR 对 30 例胃癌组织和配对的癌旁组织 (ANCTs) 进行 OIP5-AS1 和 THRIL 检测。 结果: 与 ANCTs 相比，胃肿瘤中 HULC 、 OIP5-AS1 和 THRIL 转录量显著降低 (p值分别为.02 、 0.02 和 0.007)。HULC 、 MALAT1 、 OIP5-AS1 、 PVT1 、 FAS-AS1 和 THRIL 的相对转录量与原发肿瘤的部位相关 (P 值 =。 002，分别为 0.003，0.002，0.002，0.002 和 0.001)。此外，PVT1 的相对表达水平与吸烟史相关 (p值 =.04)。确定了肿瘤样本和 ANCTs 中这些 lncrna 的转录数量之间的相关性。受试者工作特征曲线评估证明 THRIL 在所提及的 lncrna 中诊断能力最高 (曲线下面积 (AUC) = 0.72，P 值 =.001)。HULC 和 OIP5-AS1 随后排名 (AUC 值分别为 0.69 和 0.68; P 值分别为 0.005 和 0.007)。 结论: 目前的研究强调了这些转录本在胃癌标本中的失调，并提出了一些这些转录本，以进一步评估其作为癌症生物标志物的适用性。
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