rs1016860 of BCL2 3'UTR associates with hsa-miR-629-5p binding potential in breast cancer and gastric cancer in isfahan population.
BCL2 3 'utr 的 rs1016860 与伊斯法罕人群乳腺癌和胃癌中的 hsa-miR-629-5p 结合潜能相关。
- 作者列表："Doulabi MSH","Ghaedi K","Ranji N","Koohpar ZK
INTRODUCTION:Breast cancer is caused by the interaction of inherited and environmental risk factors. Also, gastric cancer is the second fatal carcinoma. B-cell leukemia/lymphoma 2 gene family plays a crucial role in carcinogenesis by inhibiting the apoptosis process. MATERIALS AND METHODS:In this study, 129 patients with breast cancer and 132 controls as well as 136 patients with gastric cancer and 50 controls were enrolled. We used Real time PCR to determine the genotype of the samples. Finally, we analyzed the diagram based on high temperature melting curve diagram using the MICPCR software, followed by bioinformatics prediction of rs1016860 functions. RESULTS:rs1016860 of BCL2 gene with CC, CT, and TT genotypes were observed in this region. The association of Estrogen receptor and Progesterone receptor, cancer stage and grade of cancer in the patients with genotypes was significant in breast cancer. The association of the status of primary tumor in the patients with genotypes is significant in gastric cancer (Chi-Square p <0.05 and p = 0.000 did not follow the Hardy-Weinberg equilibrium). DISCUSSION:It was predicted that the TT genotype could be dangerous in breast cancer and gastric cancer; it is expected via bioinformatics that this SNP could lead to signaling pathways of cancer progression, by altering the binding potential of miR-629-5p to BCL2 3'UTR.
引言: 乳腺癌是由遗传和环境危险因素相互作用引起的。此外，胃癌是第二致命的癌症。B细胞白血病/淋巴瘤 2 基因家族通过抑制细胞凋亡过程在癌变过程中起着至关重要的作用。 材料和方法: 本研究纳入了 129 例乳腺癌患者和 132 例对照，以及 136 例胃癌患者和 50 例对照。我们使用 Real time PCR 确定样本的基因型。最后，我们使用 MICPCR 软件分析了基于高温熔解曲线图的图，随后对 rs1016860 功能进行了生物信息学预测。 结果: 在该区域观察到 BCL2 基因 rs1016860 位点有 CC 、 CT 和 TT 基因型。乳腺癌患者雌激素受体和孕激素受体与癌症分期和癌症分级有显著相关性。胃癌患者原发肿瘤状态与基因型的相关性显著 (卡方 p <0.05，p = 0.000 未遵循 Hardy-Weinberg 平衡)。 讨论: 预测 TT 基因型在乳腺癌和胃癌中可能是危险的; 通过生物信息学预计该 SNP 可能导致癌症进展的信号通路,通过改变 miR-629-5p 与 BCL2 3 'utr 的结合潜能。
METHODS::In colorectal cancer (CRC), hepatic arterial infusion (HAI) chemotherapy may convert primarily unresectable CRC liver metastases (CLM) into resectability, although the risk of metastatic recurrence remains high after CLM ablation. We investigated the role of antitumour immunity invoked by first-line oxaliplatin-HAI for long-term CLM outcome. In a prospective study cohort of primarily unresectable CLM, we assessed patients' fms-related tyrosine kinase 3 ligand (FLT3LG) in serum, reflecting opportune intratumoural immune activity, at baseline and following 1-3 sequences of oxaliplatin-HAI. The end points were CLM resectability and overall survival. Patients who presented an immediate twofold increment of circulating FLT3LG during the treatment and at its completion were scored as CLM resectable (16.4% with both features), were alive at final follow-up 8-12 years later. All patients experienced FLT3LG increase during the treatment course, but those who remained unresectable or had the disease converted but presented a slow and gradual FLT3LG accretion, later died of the metastatic disease. These data provide further support to our previous findings that tumour-directed immunity invoked by oxaliplatin-containing therapy predicts excellent outcome of early advanced CRC if macroscopic tumour ablation is rendered possible by the 'classic' tumour response to the cytotoxic treatment.
METHODS::Prostate cancer is one of the primary causes of death around the world. As an important drug, flutamide has been used in the clinical diagnosis of prostate cancer. However, the over dosage and improper discharge of flutamide could affect the living organism. Thus, it necessary to develop the sensor for detection of flutamide with highly sensitivity. In this paper, we report the synthesis of lanthanum cobaltite decorated halloysite nanotube (LCO/HNT) nanocomposite prepared by a facile method and evaluated for selective reduction of flutamide. The as-prepared LCO/HNT nanocomposite shows the best catalytic performance towards detection of flutamide, when compared to other bare and modified electrodes. The good electrochemical performance of the LCO/HNT nanocomposite modified electrode is ascribed to abundant active sites, large specific surface area and their synergetic effects. Furthermore, the LCO/HNT modified electrode exhibits low detection limit (0.002 μM), wide working range (0.009-145 μM) and excellent selectivity with remarkable stability. Meaningfully, the developed electrochemical sensor was applied in real environmental samples with an acceptable recovery range.
METHODS::Several studies have indicated that cancer-associated fibroblasts (CAFs) could promote cancer progression in many malignancies. However, the mechanism by which CAFs promote the growth and metastasis of lung cancer remains poorly defined. In the present study, CAFs and normal fibroblasts (NFs) were isolated from human lung cancer and adjacent tissue. The data showed that the conditional medium (CM) of CAFs could increase the proliferation, migration and invasion of lung cancer cells. Vascular cell adhesion molecule-1 (VCAM-1) showed a higher expression in CAF-CM than NF-CM, and blocking VCAM-1 in CAF-CM attenuated the proliferation and invasion of cancer cells. Further, the results showed that VCAM-1 secreted from CAFs activated AKT and MAPK signaling via receptor α4β1 integrin (very-late antigen (VLA)-4) in lung cancer cells. Moreover, CAFs promoted VCAM-1 expression and tumor growth in vivo. Additionally, bioinformatics analysis indicated a positive correlation on the CAF marker protein alpha-smooth muscle actin (α-SMA) and VCAM-1 expression, which was associated with a poor prognosis in lung cancer patients. These findings demonstrate that the VCAM-1 secreted from CAFs enhances growth and invasion by activating the AKT and MAPK signaling of lung cancer cells.