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Combination of Gas Plasma and Radiotherapy Has Immunostimulatory Potential and Additive Toxicity in Murine Melanoma Cells in Vitro.

气体血浆和放疗联合应用在体外小鼠黑色素瘤细胞中具有免疫刺激潜力和累加毒性。

  • 影响因子:4.1830
  • DOI:10.3390/ijms21041379
  • 作者列表:"Pasqual-Melo G","Sagwal SK","Freund E","Gandhirajan RK","Frey B","von Woedtke T","Gaipl U","Bekeschus S
  • 发表时间:2020-02-18
Abstract

:Despite continuous advances in therapy, malignant melanoma is still among the deadliest types of cancer. At the same time, owing to its high plasticity and immunogenicity, melanoma is regarded as a model tumor entity when testing new treatment approaches. Cold physical plasma is a novel anticancer tool that utilizes a plethora of reactive oxygen species (ROS) being deposited on the target cells and tissues. To test whether plasma treatment would enhance the toxicity of an established antitumor therapy, ionizing radiation, we combined both physical treatment modalities targeting B16F10 murine melanoma cell in vitro. Repeated rather than single radiotherapy, in combination with gas plasma-introduced ROS, induced apoptosis and cell cycle arrest in an additive fashion. In tendency, gas plasma treatment sensitized the cells to subsequent radiotherapy rather than the other way around. This was concomitant with increased levels of TNFα, IL6, and GM-CSF in supernatants. Murine JAWS dendritic cells cultured in these supernatants showed an increased expression of cell surface activation markers, such as MHCII and CD83. For PD-L1 and PD-L2, increased expression was observed. Our results are the first to suggest an additive therapeutic effect of gas plasma and radiotherapy, and translational tumor models are needed to develop this concept further.

摘要

: 尽管治疗不断进步,恶性黑色素瘤仍然是最致命的癌症之一。同时,由于其高度的可塑性和免疫原性,黑色素瘤在测试新的治疗方法时被视为模型肿瘤实体。冷物理血浆是一种新型的抗癌工具,利用大量的活性氧 (ROS) 沉积在靶细胞和组织上。为了测试血浆治疗是否会增强已建立的抗肿瘤疗法电离辐射的毒性,我们在体外结合了两种靶向 B16F10 鼠黑色素瘤细胞的物理治疗方式。重复而不是单次放疗,结合气体血浆引入 ROS,以相加的方式诱导细胞凋亡和细胞周期阻滞。趋向于气体等离子体处理使细胞对随后的放疗敏感,而不是相反。这伴随着上清液中 tnf α 、 IL6 和 GM-CSF 水平的增加。在这些上清液中培养的鼠颌骨树突状细胞显示细胞表面活化标记物的表达增加,如 MHCII 和 cd83。对于 PD-L1 和 PD-L2,观察到表达增加。我们的结果首次提出了气体血浆和放疗的相加治疗效果,需要翻译肿瘤模型来进一步发展这一概念。

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DOI:10.1002/ijc.32847
作者列表:["Abrahamsson H","Jensen BV","Berven LL","Nielsen DL","Šaltytė Benth J","Johansen JS","Larsen FO","Johansen JS","Ree AH"]

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影响因子:4.88
发表时间:2020-03-01
DOI:10.1016/j.ecoenv.2019.110098
作者列表:["Suvina V","Kokulnathan T","Wang TJ","Balakrishna RG"]

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翻译标题与摘要 下载文献
影响因子:6.50
发表时间:2020-03-31
来源期刊:Cancer letters
DOI:10.1016/j.canlet.2019.12.039
作者列表:["Zhou Z","Zhou Q","Wu X","Xu S","Hu X","Tao X","Li B","Peng J","Li D","Shen L","Cao Y","Yang L"]

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