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RNA-seq reveal role of bovine TORC2 in the regulation of adipogenesis.

RNA-seq 揭示了牛 TORC2 在脂肪形成调控中的作用。

  • 影响因子:3.33
  • DOI:10.1016/j.abb.2019.108236
  • 作者列表:"Khan R","Raza SHA","Junjvlieke Z","Wang H","Cheng G","Smith SB","Jiang Z","Li A","Zan L
  • 发表时间:2020-02-15
Abstract

:Low intramuscular adipose tissue (marbling) continues to be challenge for improving beef quality in Chinese cattle. Highly marbled meat is very desirable; hence, methods to increase IMF content have become a key aspect of improving meat quality. Therefore, research on the mechanism of adipogenesis provides invaluable information for the improvement of meat quality. This study investigated the effect of TORC2 and its underlying mechanism on lipid metabolism in bovine adipocytes. The TORC2 gene was downregulated in bovine adipocytes by siRNA, and RNA sequencing was performed. Downregulation of TORC2 negatively affected bovine adipocyte differentiation. In addition, a total of 577 DEGs were found, containing 146 up-regulated and 376 down-regulated genes. KEGG pathway analysis revealed that the DEGs were linked with neuroactive ligand-receptor interaction pathway, calcium signaling pathway, cAMP pathway, chemokine signaling pathway and Wnt signaling pathway. Gene Ontology (GO) term analysis of the DEGs showed that down-regulation of TORC2 gene significantly suppressed the genes regulating important GO terms of adipogenesis-related processes in bovine adipocytes, especially regulation of biological activity, regulation of primary metabolic process, regulation of multicellular organismal process, cell adhesion, lipid metabolic process, secretion, chemical homeostasis, regulation of transport, cell-cell signaling, cAMP metabolic process, cellular calcium ion homeostasis, fat cell differentiation, and cell maturation. In conclusion, our results suggest that TORC2 at least in part regulates lipid metabolism in bovine adipocytes. The results of this study provide a basis for studying the function and molecular mechanism of the TORC2 gene in regulating adipogenesis.

摘要

: 低肌内脂肪组织 (大理石花纹) 仍然是提高中国牛牛肉质量的挑战。高度大理石状的肉是非常可取的; 因此,提高 IMF 含量的方法已经成为改善肉类质量的一个关键方面。因此,对脂肪形成机制的研究为改善肉品质提供了宝贵的信息。本研究探讨 TORC2 对牛脂肪细胞脂质代谢的影响及其机制。通过 siRNA 在牛脂肪细胞中下调 TORC2 基因,并进行 RNA 测序。TORC2 的下调对牛脂肪细胞分化产生负面影响。此外,共发现 577 个 DEGs,包含 146 个上调和 376 个下调基因。KEGG 通路分析发现 DEGs 与神经活性配体-受体相互作用通路、钙信号通路、 cAMP 通路、趋化因子信号通路和 Wnt 信号通路相关联。DEGs 的 Gene Ontology (GO) 术语分析表明,TORC2 基因的下调显著抑制了调控牛脂肪细胞脂肪形成相关过程中重要 GO 术语的基因, 尤其是生物活性的调节、初级代谢过程的调节、多细胞生物过程的调节、细胞粘附、脂质代谢过程、分泌、化学稳态、转运调节、细胞-细胞信号传导、 cAMP 代谢过程、细胞钙离子稳态、脂肪细胞分化和细胞成熟。总之,我们的结果表明 TORC2 至少部分调节牛脂肪细胞的脂质代谢。本研究结果为研究 TORC2 基因调控脂肪形成的功能和分子机制提供了依据。

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DOI:10.1111/jhn.12736
作者列表:["Hone M","Nugent AP","Walton J","McNulty BA","Egan B"]

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翻译标题与摘要 下载文献
影响因子:2.76
发表时间:2020-01-30
DOI:10.1152/japplphysiol.00631.2019
作者列表:["Bonomi AG","Ten Hoor GA","De Morree HM","Plasqui G","Sartor F"]

METHODS:BACKGROUND:Low cardiorespiratory fitness (CRF) increases risk of all-cause mortality and cardiovascular events. Periodic CRF assessment can have an important preventive function. OBJECTIVE:To develop a protocol-free method to estimate CRF in daily life based on heart rate (HR) and body acceleration measurements. METHODS:Acceleration and HR data were collected from 37 subjects (M=49%) while performing a standardized laboratory activity protocol (sitting, walking, running, cycling) and during a 5-days free-living monitoring period. CRF was determined by oxygen uptake (VO2max) during maximal exercise testing. A doubly-labeled water validated equation was used to predict total energy expenditure (TEE) from acceleration data. A fitness index was defined as the ratio between TEE and HR (TEE-pulse). Activity recognition techniques were used to process acceleration features and classify sedentary, ambulatory and other activity types. Regression equations based on TEE-pulse data from each activity type were developed to predict VO2max. RESULTS:TEE-pulse measured within each activity type of the laboratory protocol was highly correlated to VO2max (r from 0.74 to 0.91). Averaging the outcome of each activity-type specific equation based on TEE-pulse from the laboratory data led to accurate estimates of VO2max (RMSE: 300.0 mlO2/min or 10%). The difference between laboratory and free-living determined TEE-pulse was 3.7 ± 11% (r =0.85). The prediction method preserved the prediction accuracy when applied to free-living data (RMSE: 367 mlO2/min or 12%). CONCLUSIONS:Measurements of body acceleration and HR can be used to predict VO2max in daily life. Activity-specific prediction equations are needed to achieve highly accurate estimates of CRF.

翻译标题与摘要 下载文献
影响因子:3.76
发表时间:2020-01-31
DOI:10.1152/ajpgi.00386.2018
作者列表:["Farr S","Stankovic B","Hoffman S","Masoudpoor H","Baker C","Taher J","Dean A","Anakk S","Adeli K"]

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