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Prevention of denervated muscle atrophy with accelerated nerve-regeneration by babysitter procedure in rat facial nerve paralysis model.

在大鼠面神经麻痹模型中通过保姆程序预防神经再生加速的失神经肌萎缩。

  • 影响因子:1.36
  • DOI:10.1002/micr.30580
  • 作者列表:"Hashimoto K","Matsumine H","Osaki H","Ueta Y","Kamei W","Shimizu M","Fujii K","Niimi Y","Miyata M","Sakurai H
  • 发表时间:2020-03-31
Abstract

PURPOSE:The "babysitter" procedure is a reconstruction technique for facial nerve complete paralysis and uses the movement source from the healthy facial nerve with a cross-nerve graft. First, an end-to-side neurorrhaphy is performed between the affected facial nerve trunk and hypoglossal nerve for continuously delivering stimuli to the mimetic muscles for preventing the atrophy of mimetic muscles. Despite favorable clinical results, histological and physiological mechanisms remain unknown. This study attempted to establish a model for the "babysitter" procedure and find its efficacy in rats with facial nerve complete paralysis. MATERIALS AND METHODS:A total of 16 Lewis rats were used and divided into 2 groups; cross nerve graft (n = 8) and babysitter groups (n = 8). The facial nerve trunk was transected in both groups. Babysitter group underwent a two-stage procedure. Cross nerve graft group underwent only the transfer of nerve graft from the healthy side to affected side. The animals were assessed physiologically by compound muscle action potential (CMAP), and the regenerated nerve tissues were evaluated histopathologically at 13 weeks after surgery. RESULTS:Facial nucleus stained with retrograde tracers proved the re-innervation of affected facial muscle by the babysitter procedure. In CMAP, the amplitude of babysitter group was significantly higher than that of the cross-facial nerve graft group (p < .05). Histological examination found a significant difference in myelin g-ratio between two groups (p < .05). CONCLUSION:This study investigated the "babysitter" procedure for rat facial nerve palsy. Babysitter procedure shortened the denervation period without mimic muscle atrophy.

摘要

目的: “保姆” 手术是一种面神经完全瘫痪的重建技术,利用来自健康面神经的运动源,通过交叉神经移植。首先,在受累面神经干和舌下神经之间进行端侧神经吻合,连续向模拟肌肉传递刺激,防止模拟肌肉萎缩。尽管良好的临床结果,组织学和生理机制仍然未知。本研究试图建立 “保姆” 程序的模型,并在面神经完全瘫痪大鼠中发现其疗效。 材料和方法: 采用 Lewis 大鼠共 16 只,分为 2 组; 交叉神经移植组 (n = 8) 和保姆组 (n = 8)。两组均切断面神经干。保姆小组进行了两阶段的程序。交叉神经移植组仅将神经移植物从健侧转移到患侧。通过复合肌肉动作电位 (CMAP) 对动物进行生理评估,并在术后 13 周组织病理学评估再生神经组织。 结果: 逆行示踪剂染色的面神经核通过保姆程序证明了受累面肌的重新神经支配。在 CMAP 中,保姆组波幅明显高于跨面神经移植组 (p <.05)。组织学检查发现两组间髓鞘 g 比值有显著性差异 (p <.05)。 结论: 本研究调查了大鼠面神经麻痹的 “保姆” 程序。保姆程序缩短了去神经周期,没有模拟肌肉萎缩。

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