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Porcine Prion Protein as a Paradigm of Limited Susceptibility to Prion Strain Propagation.

猪朊病毒蛋白作为朊病毒菌株繁殖易感性有限的范例。

  • 影响因子:4.10
  • DOI:10.1093/infdis/jiz646
  • 作者列表:"Espinosa JC","Marín-Moreno A","Aguilar-Calvo P","Benestad SL","Andreoletti O","Torres JM
  • 发表时间:2020-01-09
Abstract

:Although experimental transmission of bovine spongiform encephalopathy (BSE) to pigs and transgenic mice expressing pig cellular prion protein (PrPC) (porcine PrP [PoPrP]-Tg001) has been described, no natural cases of prion diseases in pig were reported. This study analyzed pig-PrPC susceptibility to different prion strains using PoPrP-Tg001 mice either as animal bioassay or as substrate for protein misfolding cyclic amplification (PMCA). A panel of isolates representatives of different prion strains was selected, including classic and atypical/Nor98 scrapie, atypical-BSE, rodent scrapie, human Creutzfeldt-Jakob-disease and classic BSE from different species. Bioassay proved that PoPrP-Tg001-mice were susceptible only to the classic BSE agent, and PMCA results indicate that only classic BSE can convert pig-PrPC into scrapie-type PrP (PrPSc), independently of the species origin. Therefore, conformational flexibility constraints associated with pig-PrP would limit the number of permissible PrPSc conformations compatible with pig-PrPC, thus suggesting that pig-PrPC may constitute a paradigm of low conformational flexibility that could confer high resistance to the diversity of prion strains.

摘要

: 虽然已经描述了牛海绵状脑病 (BSE) 对猪和表达猪细胞朊蛋白 (PrPC) (猪 PrP [PoPrP]-Tg001) 的转基因小鼠的实验传播, 未报告猪朊病毒病的自然病例。本研究使用 PoPrP-Tg001 小鼠作为动物生物测定或作为蛋白质错误折叠循环扩增 (PMCA) 的底物,分析了 pig-PrPC 对不同朊病毒株的敏感性。选择了一组不同朊病毒株的分离株代表,包括来自不同物种的经典和非典型/Nor98 羊瘙痒症、非典型疯牛病、啮齿类动物羊瘙痒症、人类克雅氏病和经典疯牛病。生物测定证明 PoPrP-Tg001-mice 只对经典的疯牛病药剂敏感,而 PMCA 结果表明只有经典的疯牛病才能将 pig-PrPC 转化为羊瘙痒症型 PrP (PrPSc), 独立于物种起源。因此,与 pig-PrP 相关的构象灵活性约束将限制与 pig-PrPC 相容的允许 PrPSc 构象的数量, 因此,表明 pig-PrPC 可能构成了一种低构象灵活性的范式,可以赋予朊病毒菌株的多样性高抗性。

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相关文献
影响因子:3.85
发表时间:2020-01-14
来源期刊:Neurology
DOI:10.1212/WNL.0000000000008680
作者列表:["Maddox RA","Person MK","Blevins JE","Abrams JY","Appleby BS","Schonberger LB","Belay ED"]

METHODS:OBJECTIVE:To report the incidence of prion disease in the United States. METHODS:Prion disease decedents were retrospectively identified from the US national multiple cause-of-death data for 2003-2015 and matched with decedents in the National Prion Disease Pathology Surveillance Center (NPDPSC) database through comparison of demographic variables. NPDPSC decedents with neuropathologic or genetic test results positive for prion disease for whom no match was found in the multiple cause-of-death data were added as cases for incidence calculations; those with cause-of-death data indicating prion disease but with negative neuropathology results were removed. Age-specific and age-adjusted average annual incidence rates were then calculated. RESULTS:A total of 5,212 decedents were identified as having prion disease, for an age-adjusted average annual incidence of 1.2 cases per million population (range 1.0 per million [2004 and 2006] to 1.4 per million [2013]). The median age at death was 67 years. Ten decedents were <30 years of age (average annual incidence of 6.2 per billion); only 2 of these very young cases were sporadic forms of prion disease. Average annual incidence among those ≥65 years of age was 5.9 per million. CONCLUSIONS:Prion disease incidence can be estimated by augmenting mortality data with the results of neuropathologic and genetic testing. Cases <30 years of age were extremely rare, and most could be attributed to exogenous factors or the presence of a genetic mutation. Continued vigilance for prion diseases in all age groups remains prudent.

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影响因子:2.78
发表时间:2020-01-08
DOI:10.1080/07391102.2019.1708794
作者列表:["Soto P","Claflin IA","Bursott AL","Schwab-McCoy AD","Bartz JC"]

METHODS::The conformational conversion of the cellular prion protein (PrPC) to the misfolded and aggregated isoform, termed scrapie prion protein (PrPSc), is key to the development of a group of neurodegenerative diseases known as transmissible spongiform encephalopathies (TSEs). Although the conversion mechanism is not fully understood, the role of gene polymorphisms in varying susceptibilities to prion diseases is well established. In ovine, specific gene polymorphisms in PrPC alter prion disease susceptibility: the Valine136-Glutamine171 variant (Susceptible structure) displays high susceptibility to classical scrapie while the Alanine136-Arginine171 variant (Resistant structure) displays reduced susceptibility. The opposite trend has been reported in atypical scrapie. Despite the differentiation between classical and atypical scrapie, a complete understanding of the effect of polymorphisms on the structural dynamics of PrPC is lacking. From our structural bioinformatics study, we propose that polymorphisms locally modulate the network of residue interactions in the globular C-terminus of the ovine recombinant prion protein while maintaining the overall fold. Although the two variants we examined exhibit a densely connected group of residues that includes both β-sheets, the β2-α2 loop and the N-terminus of α-helix 2, only in the Resistant structure do most residues of α-helix 2 belong to this group. We identify the structural role of Valine136Alanine and Glutamine171Arginine: modulation of residue interaction networks that affect the connectivity between α-helix 2 and α-helix 3. We propose blocking interactions of residue 171 as a potential target for the design of therapeutics to prevent efficient PrPC misfolding. We discuss our results in the context of initial PrPC conversion and extrapolate to recently proposed PrPSc structures.Communicated by Ramaswamy H. Sarma.

影响因子:4.10
发表时间:2020-01-09
DOI:10.1093/infdis/jiz646
作者列表:["Espinosa JC","Marín-Moreno A","Aguilar-Calvo P","Benestad SL","Andreoletti O","Torres JM"]

METHODS::Although experimental transmission of bovine spongiform encephalopathy (BSE) to pigs and transgenic mice expressing pig cellular prion protein (PrPC) (porcine PrP [PoPrP]-Tg001) has been described, no natural cases of prion diseases in pig were reported. This study analyzed pig-PrPC susceptibility to different prion strains using PoPrP-Tg001 mice either as animal bioassay or as substrate for protein misfolding cyclic amplification (PMCA). A panel of isolates representatives of different prion strains was selected, including classic and atypical/Nor98 scrapie, atypical-BSE, rodent scrapie, human Creutzfeldt-Jakob-disease and classic BSE from different species. Bioassay proved that PoPrP-Tg001-mice were susceptible only to the classic BSE agent, and PMCA results indicate that only classic BSE can convert pig-PrPC into scrapie-type PrP (PrPSc), independently of the species origin. Therefore, conformational flexibility constraints associated with pig-PrP would limit the number of permissible PrPSc conformations compatible with pig-PrPC, thus suggesting that pig-PrPC may constitute a paradigm of low conformational flexibility that could confer high resistance to the diversity of prion strains.

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