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In vivo quantification of excitation and kilohertz frequency block of the rat vagus nerve.

大鼠迷走神经兴奋和千赫频率阻滞的体内定量。

  • 影响因子:5.13
  • DOI:10.1088/1741-2552/ab6cb6
  • 作者列表:"Pelot NA","Grill WM
  • 发表时间:2020-01-16
Abstract

OBJECTIVE:There is growing interest in treating diseases by electrical stimulation and block of peripheral autonomic nerves, but a paucity of studies on excitation and block of small diameter autonomic axons. We conducted in vivo quantification of the strength-duration properties, activity-dependent slowing (ADS), and responses to kilohertz frequency (KHF) signals for the rat vagus nerve (VN). APPROACH:We conducted acute in vivo experiments in urethane-anesthetised rats. We placed two cuff electrodes on the left cervical VN and one cuff electrode on the anterior subdiaphragmatic VN. The rostral cervical cuff was used to deliver pulses to quantify recruitment and ADS. The caudal cervical cuff was used to deliver KHF signals. The subdiaphragmatic cuff was used to record compound action potentials (CAPs). MAIN RESULTS:We quantified the input-output recruitment and strength-duration curves. Fits to the data using standard strength-duration equations were qualitatively similar, but the resulting chronaxie and rheobase estimates varied substantially. We measured larger thresholds for the slowest fibres (0.5 to 1 m/s), especially at shorter pulse widths. Using a novel cross-correlation CAP-based analysis, we measured ADS of ~2.3% after 3 min of 2 Hz stimulation, which is comparable to ADS reported for sympathetic efferents in somatic nerves, but much smaller than ADS in cutaneous nociceptors. We found greater ADS with higher stimulation frequency and non-monotonic changes in CV in select cases. We found monotonically increasing block thresholds across frequencies from 10 to 80 kHz for both fast and slow fibres. Further, following 25 s of KHF signal, neural conduction could require tens of seconds to recover. SIGNIFICANCE:The quantification of mammalian autonomic nerve responses to conventional and KHF signals provides essential information for development of peripheral nerve stimulation therapies and for understanding their mechanisms of action.

摘要

目的: 电刺激和阻断外周自主神经治疗疾病的研究日益增多,但对小直径自主神经轴突的兴奋和阻断的研究较少。我们对大鼠迷走神经 (VN) 的强度-持续时间特性、活性依赖性减慢 (ADS) 和对千赫频率 (KHF) 信号的反应进行了体内定量。 方法: 我们在乌拉坦麻醉大鼠中进行了急性体内实验。我们在左侧颈部 VN 上放置了两个袖带电极,在前膈下 VN 上放置了一个袖带电极。使用头侧颈部袖带输送脉冲以量化招募和 ADS。使用尾侧颈袖带传递 KHF 信号。采用膈下袖带记录复合动作电位 (CAPs)。 主要结果: 我们量化了投入产出招募和强度-持续时间曲线。使用标准强度-持续时间方程拟合数据在定性上相似,但产生的 chronosis 和 rheobase 估计值差异很大。我们测量了最慢光纤 (0.5 至 1 m/s) 的较大阈值,尤其是在脉冲宽度较短的情况下。使用一种新的基于交叉相关帽的分析,我们在 2Hz 刺激 3 min 后测量了 ~ 2.3% 的 ADS,这与所报道的躯体神经交感传出的 ADS 相当, 但皮肤伤害性感受器比 ADS 小得多。我们发现在选定的病例中,较大的 ADS 具有较高的刺激频率和 CV 的非单调变化。我们发现对于快速和慢速光纤,频率从 10 到 80 kHz 单调递增的块阈值。此外,在 KHF 信号 25 s 之后,神经传导可能需要几十秒才能恢复。 意义: 哺乳动物自主神经对常规和 KHF 信号反应的定量为开发周围神经刺激疗法和理解其作用机制提供了必要的信息。

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作者列表:["Battaglini L","Contemori G","Penzo S","Maniglia M"]

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影响因子:5.13
发表时间:2020-01-16
DOI:10.1088/1741-2552/ab6cb6
作者列表:["Pelot NA","Grill WM"]

METHODS:OBJECTIVE:There is growing interest in treating diseases by electrical stimulation and block of peripheral autonomic nerves, but a paucity of studies on excitation and block of small diameter autonomic axons. We conducted in vivo quantification of the strength-duration properties, activity-dependent slowing (ADS), and responses to kilohertz frequency (KHF) signals for the rat vagus nerve (VN). APPROACH:We conducted acute in vivo experiments in urethane-anesthetised rats. We placed two cuff electrodes on the left cervical VN and one cuff electrode on the anterior subdiaphragmatic VN. The rostral cervical cuff was used to deliver pulses to quantify recruitment and ADS. The caudal cervical cuff was used to deliver KHF signals. The subdiaphragmatic cuff was used to record compound action potentials (CAPs). MAIN RESULTS:We quantified the input-output recruitment and strength-duration curves. Fits to the data using standard strength-duration equations were qualitatively similar, but the resulting chronaxie and rheobase estimates varied substantially. We measured larger thresholds for the slowest fibres (0.5 to 1 m/s), especially at shorter pulse widths. Using a novel cross-correlation CAP-based analysis, we measured ADS of ~2.3% after 3 min of 2 Hz stimulation, which is comparable to ADS reported for sympathetic efferents in somatic nerves, but much smaller than ADS in cutaneous nociceptors. We found greater ADS with higher stimulation frequency and non-monotonic changes in CV in select cases. We found monotonically increasing block thresholds across frequencies from 10 to 80 kHz for both fast and slow fibres. Further, following 25 s of KHF signal, neural conduction could require tens of seconds to recover. SIGNIFICANCE:The quantification of mammalian autonomic nerve responses to conventional and KHF signals provides essential information for development of peripheral nerve stimulation therapies and for understanding their mechanisms of action.

影响因子:2.48
发表时间:2020-01-14
DOI:10.1016/j.yebeh.2019.106644
作者列表:["Liu A","Friedman D","Barron DS","Wang X","Thesen T","Dugan P"]

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